A cost effective real-time PCR for the detection of adenovirus from viral swabs

作者：Al Siyabi Turkiya; Binkhamis Khalifa; Wilcox Melanie; Wong Sallene; Pabbaraju Kanti; Tellier Raymond; Hatchette Todd F; LeBlanc Jason J^*

来源：Virology Journal, 2013, 10(1): 184.

DOI：10.1186/1743-422X-10-184

• Homogenization
• Extraction
• Real-time PCR
• Adenovirus
• Cost analysis

Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost effective strategy was developed using a combination of homogenization and heat treatment with an %26quot;in-house%26quot; real-time PCR. In 196 swabs submitted for adenovirus detection, this crude extraction method showed performance characteristics equivalent to viral DNA obtained from a commercial nucleic acid extraction. In addition, the in-house real-time PCR outperformed traditional testing strategies using virus culture, with sensitivities of 100% and 69.2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture.

• 出版日期2013-6-7