Anti-proliferative effect of biogenic gold nanoparticles against breast cancer cell lines (MDA-MB-231 %26 MCF-7)

作者：Suganya K S Uma; Govindaraju K; Kumar V Ganesh; Prabhu D; Arulvasu C; Dhas T Stalin; Karthick V; Changmai Niranjan

来源：Applied Surface Science, 2016, 371: 415-424.

DOI：10.1016/j.apsusc.2016.03.004

• Gold nanoparticles
• Mimosa pudica
• Anti-cancer activity
• Apoptosis
• Flow cytometry

Breast cancer is a major complication in women and numerous approaches are being developed to overcome this problem. In conventional treatments such as chemotherapy and radiotherapy the post side effects cause an unsuitable effect in treatment of cancer. Hence, it is essential to develop a novel strategy for the treatment of this disease. In the present investigation, a possible route for green synthesis of gold nanoparticles (AuNPs) using leaf extract of Mimosa pudica and its anticancer efficacy in the treatment of breast cancer cell lines is studied. The synthesized nanoparticles were found to be effective in killing cancer cells (MDA-MB-231 & MCF-7) which were studied using various anticancer assays (MTT assay, cell morphology determination, cell cycle analysis, comet assay, Annexin V-FITC/PI staining and DAPI staining). Cell morphological analysis showed the changes occurred in cancer cells during the treatment with AuNPs. Cell cycle analysis revealed apoptosis in G(0)/G(1) to S phase. Similarly in Comet assay, there was an increase in tail length in treated cells in comparison with the control. Annexin V-FITC/PI staining assay showed prompt fluorescence in treated cells indicating the translocation of phosphatidylserine from the inner membrane. PI and DAPI staining showed the DNA damage in treated cells.

• 出版日期2016-5-15