Alterations of Urine TGF-beta 1 and bFGF following Bladder Outlet Obstruction: A Predictor for Detrusor Contractibility?

作者：Shi Benkang^*; Zhu Yaofeng; Laudon Vincent; Ran Lingxia; Liu Yuqiang; Xu Zhishun

来源：Urologia Internationalis, 2009, 82(1): 43-47.

DOI：10.1159/000176024

• TGF-beta 1
• bFGF
• Bladder outlet obstruction
• Urine
• Detrusor contractibility

Objectives: To investigate the alterations of urine transforming growth factor-beta 1 (TGF-beta 1) and basic fibroblast growth factor (bFGF) following bladder outlet obstruction (BOO) in rats and to determine their correlation with the impaired detrusor contractibility. Methods: Wistar rats were divided into control, 2-week BOO 6-week and BOO groups. Impaired detrusor contractibility was quantified by measuring detrusor contraction force (DCF) of detrusor strip stimulated by carbachol. The enzyme-linked immunosorbent assay method was used to determine the urine levels of the 2 factors. Correlation analysis was conducted between DCF and urine levels of the 2 factors to see if there was an association between them after BOO. Results: DCF was found to be significantly lower in the 6-week BOO group than in the 2-week BOO and control groups. There is no significant difference regarding urine TGF-beta 1 between the 2-week BOO and control groups (p > 0.05). Urine TGF-beta 1 level in the 6-week BOO group was significantly higher than in the 2-week BOO (p < 0.05) and control groups (p < 0.05). There existed a negative correlation between DCF and urine TGF-beta 1 (p < 0.05). Conclusions: In an animal model, our results have suggested the potential role of urine TGF-beta 1 as a noninvasive biomarker to predict detrusor contractibility after BOO.

• 出版日期2009
• 单位山东大学