DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: A novel adjuvant and delivery system

作者：Carneiro Catarina; Correia Alexandra; Collins Tony; Vilanova Manuel; Pais Celia; Gomes Andreia C; Oliveira M Elisabete C D Real; Sampaio Paula^*

来源：European Journal of Pharmaceutics and Biopharmaceutics, 2015, 89: 190-200.

DOI：10.1016/j.ejpb.2014.11.028

• DODAB:MO liposomes
• Nanoparticles
• Adjuvant activity
• Antigen delivery systems
• Candida albicans proteins
• Liposome endocytosis
• Humoral and cellular immune responses
• Antigen protection

We describe the preparation and characterization of DODAB:MO-based liposomes and demonstrate their adjuvant potential and use in antigen delivery. Liposomes loaded with Candida albicans proteins assembled as stable negatively charged spherical nanoparticles with a mean size of 280 nm. High adsorption efficiency (91.0 +/- 9.0%) is attained with high lipid concentrations. The nanoparticles were non-toxic, avidly taken up by macrophage cells and accumulated in membrane rich regions with an internalization time of 20 min. Immunized mice displayed strong humoral and cell-mediated immune responses, producing antibodies (IgGs) against specific cell wall proteins, Cht3p and Xog1p. DODAB:MO-based liposomes loaded with C. albicans proteins have an excellent immunogenic potential and can be explored for the development of an immunoprotective strategy against Candida infections.

• 出版日期2015-1