Molecular analysis and mating behaviour of the Trichophyton mentagrophytes species complex

作者：Symoens Francoise; Jousson Olivier; Planard Chantal; Frani Marina; Staib Peter; Mignon Bernard; Monod Michel^*

来源：International Journal of Medical Microbiology, 2011, 301(3): 260-266.

DOI：10.1016/j.ijmm.2010.06.001

• Trichophyton mentagrophytes
• Arthroderma benhamiae
• Arthroderma vanbreuseghemii
• T. interdigitale
• Mating
• 28S rDNA
• ITS rDNA

Isolates of the Trichophyton mentagrophytes complex vary phenotypically. Whether the closely related zoophilic and anthropophilic anamorphs currently associated with Arthroderma vanbreuseghemii have to be considered as members of the same biological species remains an open question. In order to better delineate species in the T. mentagrophytes complex, we performed a mating analysis of freshly collected isolates from humans and animals with A. benhamiae and A. vanbreuseghemii reference strains, in comparison to internal transcribed spacer (ITS) and 28S rDNA sequencing. Mating experiments as well as ITS and 285 sequencing unambiguously allowed the distinction of A. benhamiae and A. vanbreuseghemii. We have also shown that all the isolates from tinea pedis and tinea unguium identified as T. interdigitale based on ITS sequences mated with A. vanbreuseghemii tester strains, but had lost their ability to give fertile cleistothecia. Therefore, T. interdigitale has to be considered as a humanized species derived from the sexual relative A. vanbreuseghemii.

• 出版日期2011-3