Neuroprotective Effect of Rosmarinus officinalis Extract on Human Dopaminergic Cell line, SH-SY5Y

作者：Park Se Eun; Kim Seung; Sapkota Kumar; Kim Sung Jun^*

来源：Cellular and Molecular Neurobiology, 2010, 30(5): 759-767.

DOI：10.1007/s10571-010-9502-3

• Parkinson disease
• Rosmarinus officinalis
• Dopaminergic cell
• Hydrogen peroxide
• Apoptosis

Hydrogen peroxide (H(2)O(2)) is a major Reactive Oxygen Species (ROS), which has been implicated in many neurodegenerative conditions including Parkinson's disease (PD). Rosmarinus officinalis (R. officinalis) has been reported to have various pharmacological properties including anti-oxidant activity. In this study, we investigated the neuroprotective effects of R. officinalis extract on H(2)O(2)-induced apoptosis in human dopaminergic cells, SH-SY5Y. Our results showed that H(2)O(2)-induced cytotoxicity in SH-SY5Y cells was suppressed by treatment with R. officinalis. Moreover, R. officinalis was very effective in attenuating the disruption of mitochondrial membrane potential and apoptotic cell death induced by H(2)O(2). R. officinalis extract effectively suppressed the up-regulation of Bax, Bak, Caspase-3 and -9, and down-regulation of Bcl-2. Pretreatment with R. officinalis significantly attenuated the down-regulation of tyrosine hydroxylase (TH), and aromatic amino acid decarboxylase (AADC) gene in SH-SY5Y cells. These findings indicate that R. officinalis is able to protect the neuronal cells against H(2)O(2)-induced injury and suggest that R. officinalis might potentially serve as an agent for prevention of several human neurodegenerative diseases caused by oxidative stress and apoptosis.

• 出版日期2010-7