Ammoniphilus resinae sp nov., an endospore-forming bacterium isolated from resin fragments

作者：Lin Shih Yao; Wu Yun Hsuan; Hameed Asif; Liu You Cheng; Young Chiu Chung^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(8): 3010-3016.

DOI：10.1099/ijsem.0.001137

A polyphasic approach was used to characterize an endospore-forming bacterium, designated strain CC-RT-E-T, isolated from resin fragments. Strain CC-RT-E-T was Gram-stain-variable and facultatively anaerobic, able to grow at 20-37 degrees C (optimal 30 degrees C) and pH6.0-9.0 (optimal pH 7.0). Ellipsoidal and terminal endospores were observed. The 16S rRNA gene sequence analysis of strain CC-RT-E-T showed highest sequence similarity to Ammoniphilus oxalivorans RAOx-FST (97.8 %) and Ammoniphilus oxalaticus RAOx-1(T) (97.7 %). According to the DNA-DNA hybridization, the relatedness values of strain CC-RT-E-T with Ammoniphilus oxalivorans RAOx-FST and Ammoniphilus oxalaticus RAOx-1(T) were 21% and 29 %, respectively. The DNA G+C content was 39.2 mol% and the predominant quinone system was menaquinone-7 (MK-7). The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polyamine pattern showed predominance of symhomospermidine and putrescine. The major fatty acids found in strain CC-RT-E-T were C-14:0, iso-C-15:0, anteiso-C-15:0 and C-16:1 omega 7c/C-16:1 omega 6c. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain CC-RT-E-T is considered to represent a novel species of the genus Ammoniphilus, for wh ich the name Ammoniphilus resinaesp. nov. is proposed. The type strain is CC-RT-E-T (= BCRC 80314(T) = DSM 24738(T)).

• 出版日期2016-8