科研之友
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摘要
The surface binding properties of apoferritin were investigated using two different substances, specifically, non-ionic nicotinamide and calcium. Nicotinamide could bind with apoferritin through hydrogen bonding. The binding location could be estimated by combining the Forster's non-radiation resonance energy transfer theory model and Molegro Virtual Docker docking software. The surface and internal cavities of apoferritin could be used to bind calcium through electrostatic attraction. Apoferritin monomers were stabilized only when the calcium concentration was lower than 20,000 mu M, whereas a high calcium concentration could promote serious protein aggregation.
