Determination of esmolol and metabolite enantiomers within human plasma using chiral column chromatography

作者：Fang Lei; Bykowski Jurkiewicz Crystal; Sarver Jeffrey G; Erhardt Paul W^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2010, 878(26): 2449-2452.

DOI：10.1016/j.jchromb.2010.07.006

• Esmolol
• Esmolol metabolite
• Enantiomers
• Chiral column chromatography
• LC-MS/MS
• HPLC

A high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of each of esmolol's enantiomers at the 25-1000 ng/ml concentrations observed in human plasma upon intravenous administration of this rapidly metabolized beta-adrenergic receptor blocking agent. Alternatively. a high performance liquid chromatography (HPLC) UV detection method has been developed for the simultaneous determination of each of the enantiomers for esmolol's metabolite which, in turn, achieve 2.5-50 mu g/ml concentrations in human plasma. Utilizing chiral columns, these methods do not require a precolumn asymmetric derivatization step. Linearity in all cases was >0.99. Precision and accuracy at all but the lowest concentrations were within +/- 6% for the esmolol enantiomers and within +/- 2.5% for the esmolol metabolite enantiomers. These values should be suitable for performing thorough pharmacokinetic studies for all of the stereoisomers of this prototypical soft drug and its corresponding metabolite.

• 出版日期2010-9-15