Disposable electrochemical immunosensor for Brettanomyces bruxellensis based on nanogold-reduced graphene oxide hybrid nanomaterial

作者：Borisova Boryana; Villalonga Maria L; Arevalo Villena Maria; Boujakhrout Abderrahmane; Sanchez Alfredo; Parrado Concepcion; Pingarron Jose M; Briones Perez Ana^*; Villalonga Reynaldo^*

来源：Analytical and Bioanalytical Chemistry, 2017, 409(24): 5667-5674.

DOI：10.1007/s00216-017-0505-5

• Brettanomyces bruxellensis
• Biosensor
• Graphene
• Screen printed electrode
• Red wine

The assembly of a novel disposable amperometric immunosensor for the detection of the red wine spoilage yeast Brettanomyces bruxellensis is reported. The nanostructured sensing interface was prepared by first coating carbon screen printed electrodes with a gold nanoparticles-reduced graphene oxide hybrid nanomaterial, which was then modified with 3-mercaptopropionic acid to further immobilize specific antibodies for B. bruxellensis via a carbodiimide-coupling reaction. The functionalized electrode allowed the amperometric detection of B. bruxellensis in buffered solutions and red wine samples in the range of 10-10(6) CFU/mL and 10(2)-10(6) CFU/mL, with low detection limits of 8 CFU/mL and 56 CFU/mL, respectively. The electrochemical immunosensor also exhibited high reproducibility, selectivity, and storage stability.

• 出版日期2017-9