Alternative polyadenylation of the gene transcripts encoding a rat DNA polymerase beta

作者：Konopinski R^*; Nowak R; Siedlecki JA

来源：Gene, 1996, 176(1-2): 191-195.

DOI：10.1016/0378-1119(96)00245-4

• polyadenylation
• polymerase beta
• cDNA
• beta-pol gene

Rat cells produce two different transcripts of DNA polymerase beta (beta-Pol). The low-molecular-weight transcript (1.4 kb) was already sequenced. We report here the cloning and sequencing of the full-length cDNA, corresponding to the high-molecular-weight (HMW) transcript (4.0 kb) of beta-Pol. Sequence data strongly suggest that both transcripts are produced from a single gene by alternative polyadenylation. The HMW transcript contains the entire 1.4 kb transcript sequence and the additional 2.2 kb on the 3' end. The 3' UTR of the HMW transcript contains some regulatory sequences which are not present in the 1.4-kb transcript. The A+U-rich fragment and (GU)(21) sequence are believed to influence the stability of the mRNA. The functional significance of the A-rich region locally destabilizing double-stranded secondary structure remains unknown.

• 出版日期1996-10-17