A comparison of host-defense peptides in skin secretions of female Xenopus laevis x Xenopus borealis and X. borealis x X. laevis F1 hybrids

作者：Mechkarsk Milena; Prajeep Manju; Leprince Jerome; Vaudry Hubert; Meetani Mohammed A; Evans Ben J; Conlon J Michael^*

来源：Peptides, 2013, 45: 1-8.

DOI：10.1016/j.peptides.2013.04.008

• Xenopus
• F1 hybrid
• Frog skin secretion
• Antimicrobial peptide
• Caerulein
• Xenopsin
• Allopolyploidization

Peptidomic analysis was used to compare the diversity of host-defense peptides in norepinephrine-stimulated skin secretions from laboratory-generated female F1 hybrids of Xenopus laevis and Xenopus borealis (Pipidae). Skin secretions of hybrids with maternal X. laevis (X-LB) contained 12 antimicrobial peptides (AMPs), comprising 8 from X. laevis and 4 from X. borealis. Magainin-B1, XPF-B1, PGLa-B1 CPF-B2, CPF-B3 and CPF-B4 from X. borealis and XPF-1, XPF-2, and CPF-6 from X. laevis were not detected and CPF-1 and CPF-7 were present in low concentration. The secretions contained caerulein and caerulein-B1 derived from both parents but lacked X. laevis xenopsin and X. borealis caerulein-B2. Skin secretions of hybrids with maternal X. borealis (X-BL) contained 14 AMPs comprising 6 from X. borealis and 8 from X. laevis. Magainin-B1, XPF-B1, PGLa-B1, CPF-B2, XPF-1, CPF-5, and CPF-7 were absent and CPF-B3, CPF-B4, CPF-1 and CPF-6 were present only in low concentration. Xenopsin and caerulein were identified in the secretions but caerulein-B2 was absent and caerulein-B1 was present in low concentration. No peptides were identified in secretions of either X-LB or X-BL hybrids that were not present in the parental species. The data indicate that hybridization between X. laevis and X. borealis results in increased diversity of host-defense peptides in skin secretions but point to extensive AMP gene silencing compared with previously studied female X. laevis x X. muelleri F1 hybrids and no novel peptide expression.

• 出版日期2013-7