A molecularly imprinted polymer based on multiwalled carbon nanotubes for separation and spectrophotometric determination of L-cysteine

作者：Hashemi Mahdi; Nazari Zahra; Bigdelifam Davood

来源：Microchimica Acta, 2017, 184(8): 2523-2532.

DOI：10.1007/s00604-017-2236-x

• L-Cysteine
• Molecular imprinting
• Solid-phase extraction
• Charge transfer complexation
• Multiwalled carbon nanotubes
• Spectrophotometry
• Preconcentration

The authors describe a molecularly imprinted polymer (MIP) deposited on multiwalled carbon nanotubes (MIP/MWCNTs) for separation and preconcentration of L-cysteine (L-Cys). The MIP was characterized by scanning electron microscopy, X-ray diffraction and FT-IR and via adsorption kinetics and adsorption isotherms. The MIP is shown to be a viable sorbent for L-Cys which subsequently is quantified by spectrophotometry through formation of a charge transfer complex with the DDQ reagent. The experimental parameters affecting separation efficiency and spectrophotometric determination were optimized. Under optimum conditions and at an analytical wavelength of 478 nm, the calibration plot is linear in the 4.0 to 180 ng mL(-1) concentration range, and the limit of detection (at an S/N ratio of 3) is 2.3 ng mL(-1). The intra-day and inter-day precision are in the range from 2.4 to 3.6%. The method was successfully applied to determination of L-Cys in spiked human serum and water samples where it gave recoveries ranging from 96.6 to 102.4%.

• 出版日期2017-8