Development and Validation of a Sensitive and Specific sodB-Based Quantitative PCR Assay for Molecular Detection of Ehrlichia Species

作者：Qurollo Barbara A; Riggins Dana; Comyn Alaire; Zewde Merone T; Breitschwerdt Edward B^*

来源：Journal of Clinical Microbiology, 2014, 52(11): 4030-4032.

DOI：10.1128/JCM.02340-14

We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay%26apos;s limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.

• 出版日期2014-11