Direct Electrochemistry of Hemoglobin Immobilized on Glassy Carbon Electrode Modified with Anodic Alumina Oxide Films

作者：Wu Wuai^*; Guo Mandong; Yin Zhifen; Wei Jingrui; Li Jingfang; Li Jun; Zhang Yu

来源：Acta Chimica Sinica, 2009, 67(8): 781-785.

• hemoglobin
• anodic alumina oxide films
• chemical modified electrode

A steady Hb/PPy/AAO/GC electrode was obtained by successfully immobilizing hemoglobin (Hb) on a glassy carbon (GC) electrode, which was modified using anodic alumina oxide (AAO) films. Its optimum experimental conditions were found. The electrochemistry behavior of the Hb/PPy/AAO/GC biosensor in PBS (pH=6.8) and the direct electron transfer mechanism of Hb were discussed, finding that the anodic alumina oxide films kept the biological activity of Hb. Moreover through nano-size effect of the films, the direct electron transfer between Hb and the electrode finished. The study is important to life science and clinical medicine.

• 出版日期2009-4-28
• 单位山西师范大学