Microscale fractionation facilitates detection of differentially expressed proteins in Alzheimer's disease brain samples

作者：Lopez MF^*; Mikulskis A; Golenko E; Melov S; Bennett D; Cherkasskiy A; Kuzdzal S; Kramer S; Sissors D

来源：Electrophoresis, 2004, 25(15): 2557-2563.

DOI：10.1002/elps.200406011

• fractionation
• image analysis
• mass spectrometry
• proteomics
• two-dimensional electrophoresis

Fractionation enhances the resolution of proteins with similar characteristics by reducing the number of proteins that comigrate in gels, thus facilitating the detection of lower-abundance proteins and the accurate determination of quantitative and qualitative differences in disease and normal samples. An efficient, reproducible microscale fractionation protocol for complex protein mixtures using novel ion-exchange membrane chromatographic substrates (PerkinElmer, Boston, MA, USA; Vivascience, Carlsbad, CA, USA) is described. The fractionation techniques were used in combination with two-dimensional (2-D) gels and orthogonal matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry to identify differentially expressed proteins in brain samples from persons with and without Alzheimer's disease.

• 出版日期2004-8