Multiphoton-Excited Luminescent Lanthanide Bioprobes: Two- and Three-Photon Cross Sections of Dipicolinate Derivatives and Binuclear Helicates

作者：Eliseeva Svetlana V; Auboeck Gerald; van Mourik Frank; Cannizzo Andrea; Song Bo; Deiters Emmanuel; Chauvin Anne Sophie; Chergui Majed; Buenzli Jean Claude G

来源：Journal of Physical Chemistry B, 2010, 114(8): 2932-2937.

DOI：10.1021/jp9090206

Multiphoton excited luminescent properties or water-soluble Eu(III) and Tb(III) complexes with derivatives of dipicolinic acid functionalized with a polyoxyethylene pendant arm and terminal groups, [Eu(L(OMe))(3)](3-), [Eu(L(NH2))(3)](3-), and [Tb(L(OH))(3)](3-), as well as of binuclear helicates with overall composition [Ln(2)(L(CX))(3)] (X = 2, 5) are investigated. Characteristic emission from the (5)D(0) and (5)D(4) excited levels of Eu(III) and Tb(III), respectively, upon approximate to 800 nm excitation results from three-photon absorption (3PA) for [Eu(L(OMe))(3)](3-), [Eu(L(NH2))(3)](3-), [Tb(L(OH))(3)](3-), and [Ln(2)(L(C2))(3)], while luminescence from [Eu(2)(L(C5))(3)] is induced by two-photon absorption (2PA) owing to its IPA spectrum extending further into the visible. The 3PA cross sections have been determined and are the first ones reported for lanthanide complexes: (i) those of Eu(III) and Tb(III) bimetallic helicates [Ln(2)(L(C2))(3)] are 20 times larger compared to the corresponding values for tris(dipicolinates); (ii) derivatization of dipicolinic acid for Tb(III) complexes has almost no influence on the 3PA cross section; however, for Eu(III) complexes a similar to 2 times decrease is observed. The feasibility of [Eu(2)(L(C5))(3)] its multiphoton luminescence bioprobe is demonstrated by two-photon scanning microscopy imaging experiments on HeLa cells incubated with this bimetallic helicate.

• 出版日期2010-3-4