Antimicrobial Properties and Osteogenicity of Vancomycin-Loaded Synthetic Scaffolds Obtained by Supercritical Foaming

作者：Garcia Gonzalez Carlos A; Banos Joana; Rey Rico Ana; Redondo Pablo; Gomez Amoza Jose L; Concheiro Angel; Alvarez Lorenzo Carmen; Monteiro Fernando J

来源：ACS Applied Materials & Interfaces, 2018, 10(4): 3349-3360.

DOI：10.1021/acsami.7b17375

• polymeric scaffold
• vancomycin
• supercritical CO2
• foaming
• bone regeneration

Advanced porous synthetic scaffolds are particularly suitable for regeneration of damaged tissues, but there is the risk of infections due to the colonization of microorganisms, forming biofilms. Supercritical foaming is an attractive processing method to prepare bone scaffolds, regulating simultaneously the porosity and loading of bioactive compounds without loss of activity. In this work, scaffolds made of poly-epsilon-caprolactone (50 kDa), containing chitosan and an antimicrobial agent (vancomycin); were processed by supercritical CO2 foaming for bone regeneration purposes. The obtained scaffold's showed a suitable combination Of morphological (porosity, pore size distribution, and interconnectivity), time-dependent in vitro vancomycin release behavior and biological properties (cell viability and proliferation, osteodifferentiation, and tissue-scaffold integration). The scaffolds sustained vancomycin release for more than 2 weeks. Finally, the antimicrobial activity of the scaffolds was tested against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria after 24 h of incubation with fall growth inhibition for S. aureus.

• 出版日期2018-1-31