A Substrate-Based Approach for the Selection of Oil-Bearing Heterotrophs from Nitrogen-Deficient Soil for Lipid Production

作者：Kuttiraja M; Krishna Shyam; Dhouha Ayed; Tyagi Rajeshwar Dayal^*

来源：Applied Biochemistry and Biotechnology, 2015, 175(4): 1926-1937.

DOI：10.1007/s12010-014-1378-0

• Lipid production
• Crude glycerol
• Biomass
• Biolog

In this study, nine heterotrophic yeast isolates were tested for their ability to assimilate crude glycerol and consecutive conversion to triacylglycerides (TGAs). All the organisms were initially screened on crude glycerol-based selection media, and those producing lipid globules were further evaluated for lipid production. Sudan Black B staining of eight isolates showed lipid globules. These strains were further studied at different C/N ratio. The molecular identification revealed that the isolates belonged to the genera of Yarrowia and Candida. Among these isolates, SKY7 (Yarrowia lipolytica) produced up to 42.04 +/- 0.11 % of lipid w/w) with a C/N ratio of 100 and fermentation time of 72 h. The other strains produced 5.82 +/- 0.4 to 34.57 +/- 0.44 % lipid (w/w). The GC-flame ionization detector (FID) lipid profile showed that the lipid produced by the strains had close resemblance with vegetable oil and could serve as a feedstock for biodiesel production. Biolog test of the isolates revealed a wide spectrum of carbon utilization.

• 出版日期2015-2