Anti-Diabetic Effects of Acankoreagenin from the Leaves of Acanthopanax Gracilistylus Herb in RIN-m5F Cells via Suppression of NF-kappa B Activation

作者：Lu, Man-Xia; Yang, Yang; Zou, Qin-Peng; Luo, Jiao; Zhang, Bin-Bei; Liu, Xiang-Qian^*; Hwang, Eun-Hee^*

来源：Molecules, 2018, 23(4): 958.

DOI：10.3390/molecules23040958

• Acanthopanax gracilistylus
• acankoreagenin
• anti-diabetic effects
• alpha-glucosidase
• alpha-amylase
• PTP1B
• RIN-m5F cells

Diabetes mellitus is a chronic degenerative disease that causes long-term complications and represents a serious public health problem. In this manuscript, acankoreagenin isolated from the leaves of Acanthopanax gracilistylus (LAG) is thought to possess excellent anti-diabetic properties. In vitro, anti-diabetic activities were assessed based on the inhibitory activities with alpha-glucosidase (IC50 13.01 mu M), alpha-amylase (IC50 30.81 mu M), and PTP1B (IC50 16.39 mu M). Acankoreagenin showed better anti-diabetic effects. Then, an investigation was performed to analyze the insulin secretion effects of the insulin-secreting cell line in RIN-m5F cells. It was found that acankoreagenin could increase the insulin release in RIN-m5F cells. It was also found that acankoreagenin reduced NO production, activity of caspase-3, and the reactive oxygen species levels in the cells injured by processing of cytokines. In western blotting, inactivation of NF-kappa B signaling was confirmed. Acankoreagenin (20 mu M) showed a higher I-kappa B alpha expression and lower NF-kappa B expression than the control group and showed a better expression than the positive control L-NAME (1 mM) (p < 0.05). This study demonstrates the anti-diabetic effects of acankoreagenin in vitro and suggests acankoreagenin might offer therapeutic potential for treating diabetes mellitus.

• 出版日期2018-4
• 单位湖南中医药大学; 山西大同大学