NEW MICROSATELLITE LOCI FOR PROSOPIS ALBA AND P. CHILENSIS (FABACEAE)

作者：Bessega Cecilia F^*; Pometti Carolina L; Miller Joe T; Watts Richard; Saidman Beatriz O; Vilardi Juan C

来源：Applications in Plant Sciences, 2013, 1(5): 1200324.

DOI：10.3732/apps.1200324

• Fabaceae
• microsatellite
• next-generation sequencing data
• Prosopis

Premise of the study: As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for Prosopis species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for Prosopis species from a 454 pyrosequencing run were optimized and characterized for studies in P. alba and P. chilensis. %26lt;br%26gt;Methods and Results: Twelve markers that were successfully amplified showed polymorphism in P. alba and P. chilensis. The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in P. ruscifolia, P. flexuosa, P. kuntzei, P. glandulosa, and P. pallida. %26lt;br%26gt;Conclusions: These loci will enable genetic diversity studies of P. alba and P. chilensis and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.

• 出版日期2013-5
• 单位CSIRO