Na+/HCO3 (-) Cotransporter Immunoreactivity Changes in Neurons and Expresses in Astrocytes in the Gerbil Hippocampal CA1 Region After Ischemia/Reperfusion

作者：Sohn Youdong; Yoo Ki Yeon; Park Ok Kyu; Kwon Seung Hae; Lee Choong Hyun; Choi Jung Hoon; Hwang In Koo; Seo Jeong Yeol; Cho Jun Hwi^*; Won Moo Ho

来源：Neurochemical Research, 2011, 36(12): 2459-2469.

DOI：10.1007/s11064-011-0572-5

• Transient cerebral Ischemia
• Delayed neuronal death
• Gliosis
• Neuroprotection
• 4,4 '-Diisothiocyanatostilbene-2,2 '-disulfonate

The maintenance of intracellular pH is important in neuronal function. Na+/HCO3 (-) cotransporter (NBC), a bicarbonate-dependent acid-base transport protein, may contribute to cellular acid-base homeostasis in pathophysiological processes. We examined the alterations of NBC immunoreactivity and its protein levels in the hippocampal CA1 region after transient cerebral ischemia in gerbils. In the sham-operated group, moderate NBC immunoreactivity was detected in CA1 pyramidal neurons, and, 12 h after I/R, the immunoreactivity in the pyramidal neurons was markedly increased over controls. Three days after I/R, NBC immunoreactivity nearly disappeared in the CA1 pyramidal neurons. However, NBC immunoreactivity was detected in the non-pyramidal neurons of the ischemic CA1 region at 3 days after I/R. From double immunofluorescence study with glial markers, NBC immunoreactivity was detected in astrocytes, not in microglia, at 4 days after I/R. NBC protein level in the CA1 region was significantly increased at 12 h post-ischemia and significantly decreased at 2 days post-ischemia. Thereafter, NBC protein level was again increased and returned to the level of the sham-operated group at 4 days post-ischemia. On the other hand, treatment with 4,4'-diisothiocyanatostilbene-2,2'-disulfonate (DIDS), an inorganic anion exchanger blocker including Cl-bicarbonate exchanger, protected CA1 pyramidal neurons from I/R injury at 4 days post-ischemia. These results indicate that changes in NBC expressions may play an important role in neuronal damage and astrocytosis induced by transient cerebral ischemia.

• 出版日期2011-12
• 单位河北医科大学