摘要

Bacterial blight caused by Xanthomonas oryzae pv. oryzae has been reported as one of the most destructive diseases of rice. An antagonistic strain ATC-2 against Xanthomonas oryzae pv. oryzae was screened and identified as Streptomyces termitum by biochemical and physiological assays which was reinforced by comparison of 16S rDNA sequence on Genebank via online BLAST analysis. The antimicrobial compound was isolated from the ferments broth by column chromatography over silica gel and Sephadex LH-20 and it is determined to be aloesaponarin II by H-1 NMR and EI-MS analysis. The results of antimicrobial assays revealed that aloesaponarin II possessed strong antimicrobial activity against Xanthomonas oryzae pv. oryzae with IC50 19.2 mu g/ml.