Analysis of expression profiles of long noncoding RNAs and mRNAs in brains of mice infected by rabies virus by RNA sequencing

作者：Zhao, Pingsen^*; Liu, Sudong; Zhong, Zhixiong; Jiang, Tianqi; Weng, Ruiqiang; Xie, Mengze; Yang, Songtao; Xia, Xianzhu

来源：Scientific Reports, 2018, 8(1): 11858.

DOI：10.1038/s41598-018-30359-z

• CELL-DIFFERENTIATION
• INNATE IMMUNITY
• INTERFERON
• REPLICATION
• REVEALS
• IDENTIFICATION
• EVOLUTION
• RESPONSES
• GENES
• ROLES

Rabies, caused by rabies virus (RABV), is still the deadliest infectious disease. Mechanism of host immune response upon RABV infection is not yet fully understood. Accumulating evidences suggest that long noncoding RNAs (lncRNAs) plays key roles in host antiviral responses. However, expression profile and function of lncRNAs in RABV infection remain unclear. In the present study, expression profile of lncRNAs and mRNAs profiles were investigated in RABV-infected brain tissues of mice by RNA sequencing. A total of 140 IncRNAs and 3,807 mRNAs were differentially expressed in RABV-infected animals. The functional annotation and enrichment analysis using Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that differentially expressed transcripts were predominantly involved in signaling pathways related to host immune response. The expression profiles of the selected lncRNAs in brains of mice during RABV infections were verified by quantitative real time polymerase chain reaction (qRT-PCR). To our knowledge, this is the first report to profile the lncRNA expression in RABV infected mice. Our findings provide insights into understanding the role of lncRNAs in host immune response against RABV infection.

• 出版日期2018-8-8
• 单位东北农业大学; 中国人民解放军军事医学科学院; 吉林大学; 中山大学