Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman

作者：Aguiar Jaciana S; Araujo Rosilma O; Rodrigues Maria do Desterro; Sena Kesia X F R; Batista Andre M; Guerra Maria M P; Oliveira Steno L; Tavares Josean F; Silva Marcelo S; Nascimento Silene C; da Silva Teresinha Goncalves^*

来源：International Journal of Molecular Sciences, 2012, 13(4): 4124-4140.

DOI：10.3390/ijms13044124

• Erythroxylum caatingae
• antimicrobial activity
• cytotoxic activity
• hemolytic activity
• apoptosis

In the study, we have examined the antitumor and antimicrobial activities of the methanol extract, the fractions, a fraction of total alkaloids and two alkaloids isolated from the stem of Erythroxylum caatingae Plowman. All test fractions, except the hexane fractions, showed antimicrobial activity on gram-positive bacteria and fungi. The acetate: methanol (95: 5), acetate, chloroform and hexane fractions show the highest cytotoxicity activity against the NCI-H292, HEp-2 and K562 cell lines using MTT. The absence of hemolysis in the erythrocytes of mice was observed in these fractions and 6 beta-Benzoyloxy-3 alpha-(3,4,5-trimethoxybenzoyloxy) tropane (catuabine B). Staining with Annexin V-FITC and JC-1 was used to verify the mechanism of action of the compounds of E. caatingae that showed cytotoxicity less than 30 mu g/mL in leukemic cells. After 48 h of incubation, we observed that the acetate: methanol (95: 5), acetate, and chloroform fractions, as well as the catuabine B, increased in the number of cells in early apoptosis, from 53.0 to 74.8%. An analysis of the potential of the mitochondrial membrane by incorporation of JC-1 showed that most cells during incubation of the acetate: methanol (95: 5) and acetate fractions (63.85 and 59.2%) were stained, suggesting the involvement of an intrinsic pathway of apoptosis.

• 出版日期2012-4