Voltammetry Assay for Assessment of Oxidative Stress linked Pathologies in Brain Tumor suffered Childhood Patients

作者：Pohanka Miroslav^*; Hynek David; Kracmarova Alzbeta; Kruseova Jarmila; Ruttkay Nedecky Branislav; Sochor Jiri; Adam Vojtech; Hubalek Jaromir; Masarik Michal; Eckschlager Tomas; Kizek Rene

来源：International Journal of Electrochemical Science, 2012, 7(12): 11978-11992.

• biosensor
• electrochemistry
• stress
• cancer
• medulloblastoma
• neuroblastoma
• glioblastoma
• brain cancer
• redox status

Oxidative stress plays an important role in cancers due to several reasons including metabolism disorder and hypoxia in tissue near the cancer. Numerous researchers have reported participation of oxidative stress in the cancer pathology. However, the mechanism is not well understood. In this study, we investigated oxidative stress development and depletion of antioxidants in blood samples from medulloblastoma, neuroblastoma, glioblastoma and central nervous system cancer suffered childhood patients. The blood samples were analyzed using square wave voltammetry (SWV), dimethyl-4-phenylenediamine method (DMPD), 2%26apos;-azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), free radical method (FR), ferric reducing antioxidant power (FRAP), reduced and oxidized glutathione, and metallothionein. We proved significant correlation between the markers. The findings demonstrate crucial role of oxidative stress and antioxidants levels regulation in the cancer suffered patients.

• 出版日期2012-12