科研之友
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摘要
Establishing a stable and homogeneous embryogenic cell suspension (ECS) is the prerequisite for biotechnological breeding of banana. Inducing embryogenic callus is the key step for establishing an ECS. But explants were often browning and necrotic when they were induced that decreased the possibility for getting ECS and much more labor has to be paid. In this paper, we found that browning rate of flowers cultured on 1/2 MS or 1/3 MS was lower than that of those cultured on MS basal medium. Neither activated charcoal nor pH had significant effects on decreasing flowers' browning. Flowers cultured on medium containing DTT or Na2S2O3 did not show clear superiority to those cultured on control medium. Ascorbic acid combined with 1/2 MS or 1/3 MS had much better effects than other media in controlling flowers' browning. No flowers cultured on these two media were browning and necrotic after being cultured one month. But if the dose of ascorbic acid was higher than 50 mg/L, the flowers developed slower than control. 30 mg/L of ascorbic acid was the descent concentration.
- 出版日期2013-4
- 单位中国热带农业科学院
