Antioxidant properties of xanthones from Calophyllum brasiliense: prevention of oxidative damage induced by FeSO4

作者：Blanco Ayala Tonali; Lugo Huitron Rafael; Serrano Lopez Elizabeth M; Reyes Chilpa Ricardo; Rangel Lopez Edgar; Pineda Benjamin; Noel Medina Campos Omar; Sanchez Chapul Laura; Pinzon Enrique; Cristina Trejo Solis; Silva Adaya Daniela; Pedraza Chaverri Jose; Rios Camilo; Perez de la Cruz Veronica; Torres Ramos Monica

来源：BMC Complementary and Alternative Medicine, 2013, 13: 262.

DOI：10.1186/1472-6882-13-262

• Xanthones
• Antioxidant capacity
• ROS production

Background: Reactive oxygen species (ROS) are important mediators in a number of degenerative diseases. Oxidative stress refers to the imbalance between the production of ROS and the ability to scavenge these species through endogenous antioxidant systems. Since antioxidants can inhibit oxidative processes, it becomes relevant to describe natural compounds with antioxidant properties which may be designed as therapies to decrease oxidative damage and stimulate endogenous cytoprotective systems. The present study tested the protective effect of two xanthones isolated from the heartwood of Calophyllum brasilienses against FeSO4-induced toxicity. %26lt;br%26gt;Methods: Through combinatory chemistry assays, we evaluated the superoxide (O-2(center dot-)), hydroxyl radical (OH center dot), hydrogen peroxide (H2O2) and peroxynitrite (ONOO-) scavenging capacity of jacareubin (xanthone III) and 2-(3,3-dimethylallyl)-1,3,5,6-tetrahydroxyxanthone (xanthone V). The effect of these xanthones on murine DNA and bovine serum albumin degradation induced by an OH center dot generator system was also evaluated. Additionally, we investigated the effect of these xanthones on ROS production, lipid peroxidation and glutathione reductase (GR) activity in FeSO4-exposed brain, liver and lung rat homogenates. %26lt;br%26gt;Results: Xanthone V exhibited a better scavenging capacity for O-2(center dot-), ONOO- and OH center dot than xanthone III, although both xanthones were unable to trap H2O2. Additionally, xanthones III and V prevented the albumin and DNA degradation induced by the OH center dot generator system. Lipid peroxidation and ROS production evoked by FeSO4 were decreased by both xanthones in all tissues tested. Xanthones III and V also prevented the GR activity depletion induced by pro-oxidant activity only in the brain. %26lt;br%26gt;Conclusions: Altogether, the collected evidence suggests that xanthones can play a role as potential agents to attenuate the oxidative damage produced by different pro-oxidants.

• 出版日期2013-10-11