科研之友
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摘要
Microbial sensing plays essential roles in the innate immune response to pathogens. In particular, NLRP3 forms a multiprotein inflammasome complex responsible for the maturation of interleukin (IL)-1 beta. Our aim was to delineate the role of the NLRP3 inflammasome in macrophages, and the contribution of IL-1 beta to the host defense against Citrobacter rodentium acute infection in mice. Nlrp3(-/-) and background C57BL/6 (WT) mice were infected by orogastric gavage, received IL-1 beta (0.5 mu g/mouse; ip) on 0, 2, and 4 days post-infection (DPI), and assessed on 6 and 10 DPI. Infected Nlrp3(-/-) mice developed severe colitis; IL-1 beta treatments reduced colonization, abrogated dissemination of bacteria to mesenteric lymph nodes, and protected epithelial integrity of infected Nlrp3(-/-) mice. In contrast, IL-1 beta treatments of WT mice had an opposite effect with increased penetration of bacteria and barrier disruption. Microscopy showed reduced damage in Nlrp3(-/-) mice, and increased severity of disease in WT mice with IL-1 beta treatments, in particular on 10 DPI. Secretion of some pro-inflammatory plasma cytokines was dissipated in Nlrp3(-/-) compared to WT mice. IL-1 beta treatments elevated macrophage infiltration into infected crypts in Nlrp3(-/-) mice, suggesting that IL-1 beta may improve macrophage function, as exogenous administration of IL-1 beta increased phagocytosis of C. rodentium by peritoneal Nlrp3(-/-) macrophages in vitro. As well, the exogenous administration of IL-1 beta to WT peritoneal macrophages damaged the epithelial barrier of C. rodentium-infected polarized CMT-93 cells. Treatment of Nlrp3(-/-) mice with IL-1 beta seems to confer protection against C. rodentium infection by reducing colonization, protecting epithelial integrity, and improving macrophage activity, while extraneous IL-1 beta appeared to be detrimental to WT mice. Together, these findings highlight the importance of balanced cytokine responses as IL-1 beta improved bacterial clearance in Nlrp3(-/-) mice but increased tissue damage when given to WT mice.
- 出版日期2013-12-2
