ARID1A and CEBP alpha cooperatively inhibit UCA1 transcription in breast cancer

作者：Guo, Xiao^*; Zhang, Yin; Mayakonda, Anand; Madan, Vikas; Ding, Ling-Wen; Lin, Le-Hang; Zia, Saadiya; Gery, Sigal; Tyner, Jeffrey W.; Zhou, Wu; Yin, Dong; Lin, De-Chen^*; Koeffler, H. Phillip

来源：Oncogene, 2018, 37(45): 5939-5951.

DOI：10.1038/s41388-018-0371-4

• REMODELING GENE ARID1A
• CLEAR-CELL CARCINOMA
• NONCODING RNA UCA1
• TUMOR-SUPPRESSOR
• ARID1A/BAF250A EXPRESSION
• BLADDER-CANCER
• MUTATIONS
• PROLIFERATION
• ENDOMETRIOSIS
• PROGNOSIS

As one of the primary members of SWI/SNF chromatin remodeling complexes, ARID1A contains frequent loss-of-function mutations in many types of cancers. However, the molecular mechanisms underlying ARID1A deficiency in cancer biology remain to be investigated. Using breast cancer as a model, we report that silencing ARID1A significantly increased cellular proliferation and migration. Mechanistically, primarily functioning as a transcriptional repressor, loss of ARID1A profoundly alters histone modifications and the transcriptome. Notably, ARID1A inhibited the expression of a long noncoding RNA, UCA1, by regulating chromatin access of the transcription factor CEBPa. Restoration experiments showed that UCA1 mediates the functions of ARID1A that induces loss of cellular proliferation and migration. Together, our findings characterize ARID1A as a key tumor-suppressor gene in breast cancer through cooperation with CEBPa, and loss-of-function mutations of ARID1A activates UCA1.

• 出版日期2018-11-8
• 单位丽水学院; 中山大学