A biosensor based on immobilizing horseradish peroxidase on Fe3O4 nanoparticles

作者：Yuan Yong Hai; Li Jian Ping^*

来源：Chinese Journal of Analytical Chemistry, 2007, 35(7): 1078-1082.

• magnetic nanoparticles
• horseradish peroxidase
• biosensor
• hydrogen peroxide

Firstly magnetic Fe3O4 granule was obtained by the reaction between FeSO4 and FeCl3 at 80 degrees C; Then horseradish peroxidase was covalent immobilized on Fe3O4 (about 15 nm) nanoparticles by 3-(aminopropl) triethoxysilane and glutaraldehyde. Then the magnetic properties of magnetic horseradish peroxidase particles were studied. Biosensor based on magnetic horseradish peroxidase particles attracted on the surface of solid paraffin carbon paste electrode was prepared and applied to detecte. Hydrogen peroxide. There was a linear relationship between the reduction current and the concentration of hydrogen peroxide in the concentration range of 1. 2 X 10(-7) - 8. 3 x 10(-5) mol/L, the detection limit was 4. 5 x 10(-8) mol/L. The modified electrode based on magnetic powder is renewable, more stable and cheaper, and has the virtue of good electro-catalytic property.

• 出版日期2007-7
• 单位桂林理工大学