Antinociceptive activity of Riparin II from Aniba riparia: Further elucidation of the possible mechanisms

作者：Rodrigues de Carvalho Alyne Mara; Vasconcelos Leonardo Freire; Moura Rocha Nayrton Flvio; Vasconcelos Rios Emiliano Ricardo; Dias Marlia Leite; de Franca Fonteles Marta Maria; Gaspar Danielle Macedo; Barbosa Filho Jose Maria; Chavez Gutierrez Stanley Juan; Florenco de Sousa Francisca Clea

来源：Chemico-Biological Interactions, 2018, 287: 49-56.

DOI：10.1016/j.cbi.2018.04.003

• Riparin II
• Nociception
• Pain

Riparin II (RipII) has an anti-inflammatory activity potentially due its ability to decrease TNF-alpha and IL-1 beta production and its histamine antagonism. The objective of this study was to evaluate the role of RipII in the pain process and the possible antinociceptive mechanisms involved, using classic models of nociception. Male Swiss mice were used in the assays. Determinate the acute toxicity according to the OECD 425 test guideline. The models used were the acetic acid-, formalin-, hot plate and glutamate-induced nociception. For evaluation of antinociceptive effect, the involvement of TRPV1, TRPA1, TRPM8, ASICS, Bradykinin, PKC and PKA were performed using the paw licking using agonists. The acute toxicity study did not detect any clinical signs or changes in behavior or mortality. RipII, administered orally (25 and 50 mg/kg) caused a reduction of nociception induced by acetic acid, formalin (on the second phase) and glutamate. In the investigation of antinociceptive mechanism, we used capsaicin (2.2 mu g/paw), cinnamaldehyde (10 nmol/paw), menthol (1.2 mu mol/paw), ASICS (2% acetic acid, pH 1.98) and bradykinin (10 mu g/paw). The results showed that TRPV1, TRPA1, TRPM8, ASICS and bradykinin play a role in the antinociceptive effect of RipII. The results also showed that PKA is involved too. These data demonstrate that RipII has a low or not toxicity and produced an important antinociceptive effect through mechanisms that probably involve an interaction, at least in part, TRPV1, TRPA1, TRPM8, ASICS, bradykinin and PKA participate in the RipII's antinociceptive effect.

• 出版日期2018-5-1