Nature of the genome of the saprophytic spirochete Spirochaeta aurantia and its ribosomal RNA operons

作者：McLaughlin R; Secko DM; Paul CJ; Kropinski AM^*

来源：Canadian Journal of Microbiology, 2004, 50(11): 967-971.

DOI：10.1139/W04-086

• spirochete
• pulsed-field gel electrophoresis
• ribosomal RNA
• operon
• Spirochaeta aurantia

Using restriction endonucleases DraI, AseI, and I-CeuI in conjunction with pulsed-field gel electrophoresis, we have shown that Spirochaeta aurantia M1 possesses a circular 3.98-Mb genome. This is the second largest spirochete chromosome yet analyzed. The observation that the latter enzyme cuts in 3 places suggests the presence of 3 copies of the large subunit (23S) rRNA gene (rrl), which was confirmed by Southern hybridizations. The complete sequence of 2 of the ribosomal RNA operons was determined, revealing that their structure resembled that of the typical member of the bacterial superkingdom: rrs (16S; 1561 bp), tRNA, rrl (23S; 2972 bp), and rrf (5S; 110 bp). The S. aurantia rrs-rrl intergenic regions, as with Treponema denticola, contain genes specifying a 73-nt tRNA(Ala) (anticodon TGC) and a 77-nt tRNA(Ile) (anticodon GAT).

• 出版日期2004-11