科研之友
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摘要
A bacterial strain, designated NSW-5(T), was isolated from a water sample taken from Niao-Song Wetland Park in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain NSW-5(T) were strictly aerobic, Gram-stain-negative, non-motile and polymorphic, being straight, vibrioid, curved and spiral-shaped rods surrounded by a thick capsule and forming light pink-coloured colonies. Some rings consisting of several cells were present. Growth occurred at 10-40 degrees C (optimum, 25 degrees C), with 0-3.0% NaCl (optimum, 0%) and at pH 6.0-8.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NSW-5(T) belonged to the genus Arcicella with sequence similarities of 98.6, 98.0 and 97.3% with Arcicella aquatica NO-502(T), Arcicella rosea TW5(T) and Arcicella aurantiaca TNR-18(T), respectively. The predominant cellular fatty acids were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c; 20.8 %), C-16:0 (14.6 %), iso-C-15:0 (13.8 %), C-16:1 omega 5c (12.5 %) and C-18:0 (11.4 %), and the only respiratory quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized glycolipids, aminolipids, phospholipids and aminophospholipids. The DNA G+C content of strain NSW-5(T) was 44.1 mol%. The DNA-DNA relatedness of strain NSW-5(T) with respect to recognized species of the genus Arcicella was less than 70%. On the basis of phylogenetic inference and phenotypic data, strain NSW-5(T) should be classified as a representative of a novel species, for which the name Arcicella rigui sp. nov. is proposed. The type strain is NSW-5(T) (=KCTC 23307(T)=BCRC 80260(T)). Emended descriptions of the genus Arcicella and of Arcicella aquatica, Arcicella rosea and Arcicella aurantiaca are also proposed.
- 出版日期2013-1
