An association study between the genetic polymorphisms within GnRHI, LH beta and FSH beta genes and central precocious puberty in Chinese girls

作者：Zhao, Ying; Chen, Ting; Zhou, Yuxun; Li, Kai; Xiao, Junhua^*

来源：Neuroscience Letters, 2010, 486(3): 188-192.

DOI：10.1016/j.neulet.2010.09.049

• SNP
• Linkage disequilibrium
• CPP
• LH beta
• GnRHI
• FSH beta

Objectives Gonadotropin-releasing hormone (GnRH) luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are three hypothalamic-pituitary-gonadal axis expressing hormones They play critical roles in the onset of puberty Here we report the relationship between the three hormones and Central Precocious Puberty (CPP) in Chinese Han girls Methods We analyzed the single nucleotide polymorphisms (SNPs) of 5'-flanking regions of these genes by DNA sequencing in 27 CPP samples Then the SNPs sites were genotyped by ligase detection reaction in a total of 283 Chinese Han CPP cases and 284 matched controls Distributions of the polymorphisms and haplotypes were calculated for statistical evaluation Results Nine SNPs (One in GnRHI gene -2003 C/T Five in LH beta gene -1456 C/G -1424 C/G -238 G/A -164 G/A and -34 T/A Three in FSH beta gene -1825 T/C -261 G/T and -132 T/A) were found A quantitative genetic association study was made -1825 T/C in FSH beta gene was related with CPP with a weak effect (P = 0 025) A haplotype in the 5'-flanking region of LH beta gene was significantly associated with CPP in Chinese Han girls (P = 8 25 x 10(-09)) However analysis software showed that none of SNP was found in the regulating control element of these genes Conclusions Our finding implies that the polymorphisms in the 5'-flanking regions of FSH beta gene a

• 出版日期2010-12-17
• 单位东华大学; 华东理工大学