A two-step enzymatic resolution of glycidyl butyrate

作者：Yu, Dahai; Wang, Lei; Gu, Qiang; Chen, Peng; Li, Yan; Wang, Zhi^*; Cao, Shugui

来源：Process Biochemistry, 2007, 42(9): 1319-1325.

DOI：10.1016/j.procbio.2007.06.012

• two-step
• resolution
• glycidyl butyrate
• porcine pancreatic lipase
• Novozym 435

A two-step enzymatic resolution process for production of (R)- and (S)-glycidyl butyrate was investigated and the lipases were screened. The first step involved a hydrolysis of (R,S)-glycidyl butyrate catalyzed by porcine pancreatic lipase (S-favored) with an E of 21 for production of (R)glycidyl butyrate (13.2 mmol, 98% ee, 36% yield) under the optimal conditions (pH 7.4, 30 degrees C, 30 mg/ml CTAB). Then, the recovered (R)enriched glycidol (19.8 mmol, 65% ee, 56% yield) was used for transesterification catalyzed by Novozym 435 (R-favored) with an E of 69 to obtain (S)-glycidyl butyrate (15.1 mmol, 98% ee, 42% yield) under the optimum conditions (a(w) = 0.24, n-heptane, 80 min).

• 出版日期2007-9
• 单位吉林大学