Antibody response to the 45 kDa Candida albicans antigen in an animal model and potential role of the antigen in adherence

作者：Bujdakova Helena^*; Paulovicova Ema; Borecka Melkusova Silvia; Gasperik Juraj; Kucharikova Sona; Kolecka Anna; Lell Claudia; Jensen Dorthe B; Wuerzner Reinhard; Chorvat Dusan Jr; Pichova Iva

来源：Journal of Medical Microbiology, 2008, 57(12): 1466-1472.

DOI：10.1099/jmm.0.2008/001479-0

The Candida antigen CR3-RP (complement receptor 3-related protein) is supposed to be a 'mimicry' protein because of its ability to bind antibody directed against the alpha subunit of the mammalian CR3 (CD11b/CD18). This study aimed to (i) investigate the specific humoral isotypic response to immunization with CR3-RP in vivo in a rabbit animal model, and (ii) determine the role of CR3-RP in the adherence of Candida albicans in vitro using the model systems of buccal epithelial cells (BECs) and biofilm formation. The synthetic C. albicans peptide DINGGGATLPQ corresponding to 11 amino-acids of the CR3-RP sequence DINGGGATLPQALXQITGVIT, determined by N-terminal sequencing, was used for immunization of rabbits to obtain polyclonal anti-CR3-PR serum and for subsequent characterization of the humoral isotypic response of rabbits. A significant increase of IgG, IgA and IgM anti-CR3-RP specific antibodies was observed after the third (P<0.01) and the fourth (P<0.001) immunization doses. The elevation of IgA levels suggested peptide immunomodulation of the IgA1 subclass, presumably in coincidence with Candida epithelial adherence. Blocking CR3-RP with polyclonal anti-CR3-RP serum reduced the ability of Candida to adhere to BECs, in comparison with the control, by up to 35% (P<0.001), and reduced biofilm formation by 28% (P<0.001), including changes in biofilm thickness and integrity detected by confocal laser scanning microscopy. These properties of CR3-RP suggest that it has potential for future vaccine development.

• 出版日期2008-12