科研之友
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摘要
Micropatterned surfaces with interlaced cell-friendly and cell-repellent features have been explored for applications in the scaffolds of tissue engineering and cell-based sensors by controlling the domains of protein adsorption and cell growth. We applied microcontact printing technique incorporated with a phosphorylcholine-ended disulfide (DSPC) as a novel anti-biofouling coating to fabricate micropatterns by impressing a poly(dimethyl siloxane) (PDMS) stamp. The PDMS stamp can transfer mercaptoalkylcarboxylates onto the gold-coated surfaces as the protein-favoring areas prior to the pattern being chemically adsorbed the DSPC as the protein-repellant areas. The interlaced patterns with different widths of ruled lines exhibited unique characters for cell adhesion. The properties of each self-assembled monolayer for adsorbing proteins were evaluated by quartz crystal microbalance (QCM). Preliminary results illustrated the phosphorylcholine (PC) surfaces having relative low adsorption amount toward albumin, fibrinogen, and fibronectin, yielding enhanced pattern images for scanning electron microscopy (SEM) and optical microscopy (OM). As the line width decreased from 35 mu m to 2 mu m, adherent platelets spread out along the ruled boundary of the pattern. The PC-striped areas adsorbed some isolated but round platelets, which platelets adhered to the COOH-striped areas and spread out along the borders of these areas. The 2-mu m line spacing of pattern was unable to limit the bridging phenomenon between adhesion cells. The 3T3 fibroblasts attached onto the patterns with orientation, and their pseudopods protruded and grew along the pattern axis after 24 hr of incubation, giving a high potential for cell-guiding scaffolds in tissue engineering.
- 出版日期2009
