Associations between circulating IgG antibodies to Apolipoprotein B100-derived peptide antigens and acute coronary syndrome in a Chinese Han population

作者：Hu, Weina; Zhang, Xueying; Han, Yunan; Wang, Yong; Lei, Mingming; Megson, Ian L.; Wei, Jun; Jin, Yuanzhe^*

来源：Bioscience Reports, 2018, 38(6): BSR20180450.

DOI：10.1042/BSR20180450

• LOW-DENSITY-LIPOPROTEIN
• OXIDIZED LDL
• ADAPTIVE IMMUNITY
• ATHEROSCLEROSIS
• AUTOANTIBODIES
• B-100
• DISEASE
• OXLDL
• RISK
• RESPONSES

Objectives: Acute coronary syndrome (ACS) is the major cause of mortality worldwide and caused mainly by atherosclerosis of coronary arteries. Apolipoprotein B100 (ApoB100) is a major component of low-density lipoprotein (LDL) and its oxidation can trigger inflammation in vascular endothelial cells leading to atherosclerosis. The association between antibodies to ApoB100-derived antigens and atherosclerotic diseases has been studied in recent years, but the findings appear to be controversial. The present study developed an ELISA in-house with ApoB100-derived peptide antigens to circulating anti-ApoB100 IgG antibodies in patients with ACS. Methods: Fifteen ApoB100-derived peptide antigens (Ag1-Ag15) were designed to develop an in-house ELISA for the detection of circulating anti-ApoB100 IgG levels in 350 patients with ACS and 201 control subjects amongst a Chinese population. Binary logistic regression was applied to examine the differences in anti-ApoB IgG levels between the patient group and the control group with adjustment for a number of confounding factors; the correlation between anti-ApoB100 IgG levels and clinical characteristics was also tested. Results: Patients with ACS had significantly higher levels of plasma IgG for Ag1 (adjusted P < 0.001) and Ag10 antigens (adjusted P < 0.001). There was no significant increase in the levels of IgG to the other 13 antigens in these ACS patients. In the control group, anti-Ag10 IgG levels were positively correlated with age, high-density lipoprotein (HDL), and ApoA levels (P = 0.001 for all) and negatively correlated with blood triglyceride (TG) (P = 0.008); in the patient group, anti-Ag10 IgG levels were positively correlated with LDL (P = 0.003), and negatively correlated with ApoA (P = 0.048) and systolic blood pressure (SBP) (P = 0.036). The area under ROC (receiver operator characteristic) curve (AUC) was 0.612 (95% confidence interval (CI): 0.560-0.664; P < 0.001) in anti-Ag1 IgG assay and 0.621 (95% CI: 0.569-0.672; P < 0.001) in anti-Ag10 IgG assay. Conclusion: Circulating IgG for ApoB100-derived peptide antigens may be a useful biomarker of ACS, although anti-ApoB IgG levels were not associated with the coronary artery plaque burden characterized by the coronary Gensini score.

• 出版日期2018-12-21
• 单位中国医科大学