Measuring protein reduction potentials using N-15 HSQC NMR spectroscopy

作者:Taylor Samantha L; Crawley Snowdon Harriet; Wagstaff Jane L; Rowe Michelle L; Shepherd Mark; Williamson Richard A*; Howard Mark J
来源:Chemical Communications, 2013, 49(18): 1847-1849.
DOI:10.1039/c3cc38952a

摘要

NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple N-15 HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 confirms this protein as an oxido-reductase of comparable or greater reducing strength than PDI a.

  • 出版日期2013