科研之友
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摘要
Kiwifruit is adapted to Ramsar region (north of Iran) with high relative humidity. Twenty somaclone lines were collected and transplanted from Ramsar to Sahneh region (west of Iran) with moderate and low relative humidity. After five years of transplantation, only one clone acclimatized to new conditions, grown and set fruit and seed successfully. Current study was focused on ecological adaptation related proteins and factors (such as leaf proline, soluble leaf sugar, chlorophyll a and b and relative water content) in this line and comparison with stock plant in Ramsar. This research was conducted to compare electerophoretic protein patterns and main physiological traits in kiwifruit from Ramsar and Sahneh. Results of SDS-PAGE and two-dimensional electrophoresis showed maximal and minimal differences for leaf and seed proteins, respectively. Actinidin, as main protease of kiwifruit, is expressed in fruit of Ramsar line and leaves of Sahneh kiwifruit line mainly. Its expression was relatively low in leaves of Ramsar plant and fruit of Sahneh line. Statistical analysis showed significant differences between kiwifruit of Sahneh and its stock plant in Ramsar for proline, sugar, total protein (of seed, fruit and leaf) and chlorophyll content. Correlation between sugar and proline content was positive and significant (0.931). Total protein of leaf and fruit correlated with proline significantly and negatively (0.879 and 0.835, respectively). It is concluded that protein expression in kiwifruits was affected by environmental conditions seriously. Switch on and off and down or up regulating of some proteins may be in adaptation process.
- 出版日期2012-1
