Neuroprotective effects of p53/microRNA-22 regulate inflammation and apoptosis in subarachnoid hemorrhage

作者：Yu, Shui; Zeng, Yi-Jun; Sun, Xiao-Chuan^*

来源：International Journal of Molecular Medicine, 2018, 41(4): 2406-2412.

DOI：10.3892/ijmm.2018.3392

• p53
• microRNA-22
• subarachnoid hemorrhage
• inflammation
• apoptosis

The present study aimed to investigate whether the neuroprotective effects of p53/microRNA-22 regulate inflammation and apoptosis in subarachnoid hemorrhage (SAH). In a mouse model of SAH, microRNA-22 expression was upregulated. In addition, downregulation of microRNA-22 in HEB cells increased the mRNA expression levels of interleukin (IL)-6, induced cysteine rich angiogenic inducer 61 (Cyr61) expression, and suppressed the protein expression levels of B-cell lymphoma 2-associated X protein (Bax) and caspase-3 activity. Treatment with the p53 inhibitor, pifithrin-, suppressed p53 protein expression, increased IL-6 mRNA expression, decreased microRNA-22 expression, Bax protein expression and caspase-3 activity, and induced Cyr61 expression in mice with SAH. Furthermore, p53 expression was knocked down using p53 small interfering RNA, which suppressed microRNA-22 expression and increased IL-6 mRNA expression, inhibited Bax protein expression and caspase-3 activity, and induced Cyr61 expression in HEB cells. The present study demonstrated that the neuroprotective effects of p53/microRNA-22 may regulate inflammation and apoptosis in SAH. Reverse transcription quantitative polymerase chain reaction (qPCR) was used to analyze the expression of microRNA-22, western blot analysis was used to analyze the protein expression of Bax and Cyr61.

• 出版日期2018-4
• 单位重庆医科大学