The impact of hydrogen sulfide (H2S) on neurotransmitter release from the cat carotid body

作者：Fitzgerald Robert S^*; Shirahata Machiko; Chang Irene; Kostuk Eric; Kiihl Samara

来源：Respiratory Physiology & Neurobiology, 2011, 176(3): 80-89.

DOI：10.1016/j.resp.2011.01.010

• Hydrogen sulfide
• Carotid body
• Glomus cell
• ATP
• Acetylcholine
• K-ATP channel
• Neuroprotection

Do cat carotid bodies (CBs) increase their release of acetylcholine and ATP in response to H2S? Two CBs, incubated in a Krebs Ringer bicarbonate solution at 37 degrees C, exhibited a normal response to hypoxia-increased release of acetylcholine (ACh) and ATP. They were challenged with several concentrations of Na2S, an H2S donor. H2S, a new gasotransmitter, is reported to open K-ATP channels. Under normoxic conditions the CBs reduced their release of ACh and ATP below control values. They responded identically to pinacidil, a well-known K-ATP channel opener. CB glomus cells exhibited a positive immunohistochemical signal for cystathione-beta-synthetase, a H2S synthesizing enzyme, and for a subunit of the K-ATP channel. The data suggest that Na2S may have opened the glomus cells' K-ATP channels, hyperpolarizing the cells, thus reducing their tonic release of ACh and ATP. Since during hypoxia H2S levels rise, the glomus cells responding very actively to hypoxia may be protected from over-exertion by the H2S opening of the K-ATP channels.

• 出版日期2011-5-31