The effects of non-thermal atmospheric pressure plasma jet on cellular activity at SLA-treated titanium surfaces

作者：Lee Eun Jung; Kwon Jae Sung; Uhm Soo Hyuk; Song Doo Hoon; Kim Yong Hee; Choi Eun Ha; Kim Kyoung Nam^*

来源：Current Applied Physics, 2013, 13: S36-S41.

DOI：10.1016/j.cap.2012.12.023

• Atmospheric pressure plasma jet
• Cellular activity
• Dental implant
• Hydrophilicity
• SLA-treated titanium

Titanium (Ti) is a primary material used for dental implants, and the sandblasted, large-grit, and acid-etched (SLA) surface treatment is commonly used on titanium to promote early osseointegration. Despite their benefits, SLA-treated Ti surfaces are disadvantaged by their hydrophobic property, and much research has been performed to address this problem. In current study, we hypothesized the effects of a nitrogen-based non-thermal atmospheric pressure plasma jet (NTAPPJ) treatment on SLA-treated Ti would increase hydrophilicity and cellular activity. Samples with and without the NTAPPJ treatment were analyzed using a field emission scanning electron microscope, optical surface roughness system, X-ray photoelectron spectroscope, and water contact angle measuring system. MC3T3-E1 cells (murine osteoblastic cell line) attachments and proliferations were examined by the MTT and BrdU colorimetry assay. Cell immunofluorescent microscopic images were observed by a confocal laser scanning microscope for a morphological analysis. This study found that the nitrogen-based NTAPPJ treatment on SLA-treated Ti significantly increased the hydrophilicity and MC3T3-E1 cell attachments and proliferations. Hence, it was concluded that an additional procedure of nitrogen-based NTAPPJ treatment just prior to implantation of SLA-treated Ti into oral defect sites could improve the success of dental implant surgery.

• 出版日期2013-3