Near-infrared fluorescence imaging-guided surgery improves recurrence-free survival rate in novel orthotopic animal model of head and neck squamous cell carcinoma

作者：Atallah Ihab^*; Milet Clement; Henry Maxime; Josserand Veronique; Reyt Emile; Coll Jean Luc; Hurbin Amandine; Righini Christian Adrien

来源：Head and Neck-Journal for the Sciences and Specialties of the Head and Neck, 2016, 38(S1): E246-E255.

DOI：10.1002/hed.23980

• head and neck squamous cell carcinoma
• orthotopic animal model
• near-infrared optical imaging-guided surgery
• alpha(v)beta(3) integrin
• surgical margins

Background. Appropriate animal models are required to test novel therapeutics for head and neck squamous cell carcinoma (HNSCC) such as near-infrared (NIR) imaging-guided surgery.
Methods. We developed an optimized animal model of orthotopic HNSCC (in female athymic NMRI (Naval Medical Research Institute) nude mice) with a prolonged survival time. Resection of the orthotopic tumors was performed 30 days after implantation with or without the aid of a miniaturized clinical grade NIR optical imaging device, after systemic administration of a fluorescent RGD-based probe that targets alpha(v)beta(3) integrin.
Results. NIR optical imaging-guided surgery increased the recurrencefree survival rate by 50% through the detection of fluorescent cancer residues as small as 185 mm; these fragments could remain unidentified if resection was performed exclusively under unaided visual guidance.
Conclusion. NIR optical imaging-guided surgery showed an improved HNSCC tumor resection quality in our optimized orthotopic animal model.

• 出版日期2016-4