A whole-genome radiation hybrid panel for goat

作者：Du, X. Y.; Womack, J. E.; Owens, K. E.; Elliott, J. S.; Sayre, B.; Bottcher, P. J.; Milan, D.; Podesta, M. Garcia; Zhao, S. H.^*; Malek, M.

来源：Small Ruminant Research, 2012, 105(1-3): 114-116.

DOI：10.1016/j.smallrumres.2011.11.023

• Radiation hybrid panel
• Goat
• Gene mapping
• CHRH5000

A 5000 rad goat-hamster panel of 121 whole-genome radiation hybrids was generated and preliminarily characterized. A normal diploid fibroblast culture from a male Boer goat was fused with a recipient thymidine kinase-deficient hamster cell line. The generated 121 radiation hybrids were grown and produced an average of 8.4 mg of DNA per radiation hybrid. A SINE-PCR test showed that almost all radiation hybrids retained goat DNA. The retention frequencies of the 121 hybrids were preliminarily estimated using a collection of 42 unlinked molecular markers. An optimized panel of therein 90 radiation hybrids (CHRH5000) with an average retention frequency of 34.2% was screened on the basis of interspersed repetitive DNA content and chromosome retention frequency. The development of this WG-RH panel will provide a fundamental tool for advanced goat genome mapping studies and for mammalian comparative mapping.

• 出版日期2012-6
• 单位华中农业大学