Antiproliferative effect of Aurora kinase targeting in mesothelioma

作者：Crispi Stefania; Fagliarone Claudia; Biroccio Annamaria; D'Angelo Carmen; Galati Rossella; Sacchi Ada; Vincenzi Bruno; Baldi Alfonso^*; Verdina Alessandra

来源：Lung Cancer, 2010, 70(3): 271-279.

DOI：10.1016/j.lungcan.2010.03.005

• Mesothelioma
• Aurora kinase
• Related genes
• Potential target
• Inhibitor
• ZM447439

The Aurora proteins are a small family of serine/threonine kinase that function in various stages of mitosis. Current interest in Aurora kinase relates to its role in tumours, and its potential as a therapeutic target. In this work we studied the expression of Aurora kinases A and B and related genes in human mesothelioma tissues and in five mesothelioma cell lines. Moreover, we analyzed the effects of ZM447439 (ZM), an Aurora kinase inhibitor, on cellular growth. Results evidenced an over-expression of Aurora kinase A and related genes in human mesothelioma tissues and an over-expression of Aurora kinases A and B in all cell lines. Moreover, we demonstrated that ZM447439 was able to inhibit cell growth in all cell lines and that this inhibition was due to a specific effect as demonstrated by the reduction in the level of Histone H3 phosphorylation. Our findings support a role of Aurora kinase in mesothelioma and the possibility of using Aurora kinase inhibitors in therapeutic modalities.

• 出版日期2010-12