Multimodal silica-shelled quantum dots: Direct intracellular delivery, photosensitization, toxic, and microcirculation effects

作者：Bakalova Rumiana^*; Zhelev Zhivko; Aoki Ichio; Masamoto Kazuto; Mileva Milka; Obata Takayuki; Higuchi Makoto; Gadjeva Veselina; Kanno Iwao

来源：Bioconjugate Chemistry, 2008, 19(6): 1135-1142.

DOI：10.1021/bc700431c

In the present study, we describe a multimodal QD probe with combined fluorescent and paramagnetic properties, based on silica-shelled single QD micelles with incorporated paramagnetic substances [tris(2,2,6,6-tetramethyl-3,5-heptanedionate)/gadolinium] into the micelle and/or silica coat. The probe was characterized with high photoluminescence quantum yield and good positive MRI contrast, low cytotoxicity, and easy intracellular delivery in viable cells. The intravenous administration of the probe in experimental animals did not affect significantly the physiological parameters and microcirculation (e.g., heart rate, blood pressure, diameter and shape of blood vessels), which makes it appropriate for tracing of blood circulation and in vivo multimodal imaging using fluorescent confocal microscopy, two-photon microscopy, and MRI.

• 出版日期2008-6