Antineoplastic activity of metformin on the A549 human lung carcinoma cell line in vitro

作者：Dong, Xi-Lin^*; Yang, Yuan-Li; Sun, Bao-Ni; Liu, Yan-Qin; Zhou, Jing

来源：International Journal of Clinical and Experimental Medicine, 2017, 10(7): 10711-10717.

• Metformin
• cisplatin
• lung carcinoma
• proliferation
• apoptosis

Background: To investigate the effect of metformin on proliferation of the A549 human lung carcinoma cell line and explore the possible mechanisms involved. Methods: A549 cells were exposed to metformin alone or in combination with cisplatin. Cell proliferation was measured by the MTT assay. Cell apoptosis and cell cycle stages were detected through flow cytometry. The Protein expression levels of Bcl-2 were evaluated through Western Blot analysis. Results: Metformin time- and dose-dependently inhibited the growth of A549 cells. Metformin potentiated the effect of cisplatin in vitro. Flow cytometric analysis showed that metformin can induce apoptosis in A549 cells, and arrest cell cycle at the G0/G1 phase. Western blot analysis demonstrated that metformin reduced the protein expression level of Bcl-2. Conclusion: Metformin significantly suppresses the growth of A549 cells and enhances cisplatin-induced cytotoxicity. Additional preclinical studies are being conducted to determine the applicability of metformin in the treatment of lung cancer.

• 出版日期2017
• 单位西安交通大学