Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

作者：Kobayashi Kyo; Maehata Yojiro; Kawamura Yosuke; Kusubata Masashi; Hattori Shunji; Tanaka Keisuke; Miyamoto Chihiro; Yoshino Fumihiko; Yoshida Ayaka; Wada Takahashi Satoko; Komatsu Tomoko; Takahashi Shun Suke; Lee Masaichi Chang Il^*

来源：Journal of Pharmacological Sciences, 2011, 116(1): 97-106.

DOI：10.1254/jphs.10321FP

• singlet oxygen
• hydroxyl radical
• photo-aging
• human fibroblast
• ultraviolet ray A (UVA) irradiation

In the present study, we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (P-NCP) on reactive oxygen species (ROS) such as hydroxyl radical (HO(center dot)), superoxide anion radical (O(2)(center dot-)), and singlet oxygen ((1)O(2)), and the effects on cell viability after ultraviolet ray (UV) irradiation of human fibroblasts. We confirmed, using electron spin resonance, that P-NCP directly inhibited HO(center dot) and (1)O(2). Furthermore, addition of P-NCP to fibroblasts inhibited cell death induced by UVA (400 - 315 nm) irradiation in a dose-dependent manner. In addition, the antioxidant effect on (1)O(2) was observed in the peptide fractions rich in Gly, Pro, Hyp, Glu, Ala, and Arg. We found that Gly, Hyp, Glu, and Ala directly scavenged (1)O(2). These results indicated that a peptide sequence including Gly, Hyp, Glu, and Ala could play a key role in the antioxidant effects of P-NCP on (1)O(2). It was suggested that P-NCP can inhibit photo-aging related to ROS owing to its antioxidant effects.

• 出版日期2011-5